There are numberous reports in kaffir lime’s (C. hystrix) antimicrobial activity. The crude ethnolic
extracts of kaffir lime peel showed the broadest antibacterial activity by inhibiting growth of 20 serotypes of
Salmonella and 5 species of other enterobacteria including S. Agona, S. Anatum, S. Choleraesuis, S. Derby, S.
Enteritidis, S. Lexington, S. London , S. Newport, S. Newport, S. Senftenberg , S. Virchow , S. Weltevreden , S.
Typhimurium DT104, S. Typhimurium non-DT104, S. Amsterdam, S. Hardar, S. Orion, S.panama S.
Schwarzengrund , S. Stanley, C. freundii, E. aerogenes , E. coli, K. pneumoniae , and S. marcescens . While
the crude ethnolic extracts of kaffir lime leave also showed the broadest antibacterial activity same as the
crude ethnolic extracts of kaffir lime peel, expect only S. marcescens . The essential oils of kaffir lime peel
against all 20 serotypes of Salmonella and 5 species of other enterobacteria while the essential oils of of kaffir
lime leave aginast all most tested microorganisms expect S. Newport, S. Newport , S. Newport, and E.
aerogenes . In general, the inhibitory activity of essential oils was greater than that of ethanolic extracts,
especially in kaffir lime peels . Among the serotypes of Salmonella tested, the ethanolic extracts and oils
exhibited slightly different degree of inhibition. S. Typhimurium (non-DT104 strain) was the most susceptible
serotype to both oils and extracts of kaffir lime’s peels *84+. The use of pressurized hot water extraction on
kaffir lime fruit peel and found out that when increase temperature in extraction the phenolic compound
content increasing . The ethyl acetate extract of kaffir peel showed broad spectrum of inhibition against all
Gram-positive bacteria, yeast and molds including S. aureus, B. cereus, L. monocytogenes, S. cerevisiae var.
sake and A. fumigatus TISTR 3180 . The kaffir lime leaf oil and kaffir lime peel oil were both effective
against all the pathogens were both effective against all the pathogens including 411 clinical isolates obtained
from patients with respiratory tract infections ; A. baumannii (50 isolates), Groups A (61 isolates), B (27
isolates), C (4 isolates), F (3 isolates), G streptococci (11 isolates), Haemophilus influenzae (52 isolates), M.
catarrhalis (52 isolates), methicillin-resistant S. aureus (MRSA; 50 isolates), methicillin sensitive S. aureus
(MSSA; 50 isolates) and S. pneumoniae (51 isolates) . The crude ethanolic extracts of kaffir lime leaf at 10 %
showed significantly higher inhibition than at other concentrations against A. flavus . The kaffir lime leaf
essential oils showed the strongest antibacterial activity with inhibition zones of more than 20 mm against all
5 strains of Propionibacterium acnes (DMST No. 14916, 14917, 14918, 21823, 21824) . The kaffir lime
essential oils also showed antibacterial activity against all 5 strains of P.acnes as well .